NB 6-2 generates a complex arrangement of local and intersegmental interneurons.

At late stage 15/early stage 16, intersegmental interneurons have not yet fully extended their axons.

NB 6-2 at late stage 15,

or early stage 16.

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.