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Transgenic Mouse Facility tmf
image provided by ISTT
The Transgenic Mouse Facility, at the University of Oregon (UO), is a state-of-the-art transgenic mouse core facility. It provides a technology resource to the local and regional research community to support the generation and maintenance of genetically modified mouse lines. The facility offers expertise in a variety of technical procedures, including pronuclear and blastocyst injections, a tissue culture laboratory for the manipulation of mouse Embryonic Stem (ES) cells, cryopreservation, and genotyping. The TMF also operates a transgenic barrier holding facility. The facility is fully equipped for microinjections, microsurgery, cryopreservation, gene targeting and tissue culture as well as standard molecular techniques. The TMF staff provides expertise, materials and training on a fee-for-service basis. Principal investigators who request services are required to provide a rationale for each project and to obtain IACUC approval.

 

The TMF offers the following procedures: pronuclear and blastocyst injections, ES cell culture, rederivation and cryopreservation of existing mouse strains, tail biopsy and genotyping.